Diesel Fuel Polish­ing

Liqui-Cult Fuel Test Kit (AA-0844)

Liqui-CultTM test kits are used to detect microbial growth in fluids (case of 10 test bottles)


SKU FTT01005 Category Tags , ,


Liqui-CultTM test kits are an inexpensive Do-It-Yourself method to detect microbial and fungal growth in fluids in the laboratory and in the workplace.  Areas of application include metalworking fluids, circulating cooling and process water, hydrocarbons (diesel fuel), and other liquids such as cleaners, cosmetics, liquid soaps, detergents, etc.

Liqui-CultTM testing determines the type and degree of infestation usually measured by the more expensive plate count system.  To use, simply fill the 5 cc syringe (included in your kit) with the fluid to be tested.  Inject the complete 5 cc’s through the rubber cap of the test bottle containing the liquid agar and shake it vigorously.  Incubate for 30 hours for bacterial growth or 72 hours for fungal growth. Simply compare your sample’s color after incubation to sample pictures to determine the degree of bacterial & fungal growth.

Package contains one case of 10 test bottles, 10 syringes and instructions.

Additional information

Weight 3 lbs

Complete Directions

  • Capture a fuel sample in a clean dry container.

  • Assemble syringe by first removing plunger nozzle guard. Take cap, not guard, off needle and press into plunger nozzle, leaving needle guard in place until ready to use.

  • Remove needle guard and insert tip into liquid to be tested. Draw plunger back, filling syringe with complete 5 cc’s of fluid.

  • Inject needle through rubber cap on bottle and push down on syringe plunger, emptying contents completely into it. Remove syringe from bottle, replacing needle guard and discard according to local, state and federal guidelines. Shake bottle vigorously to mix fluids.

  • Incubate bottle at room temperature for 30 hours for microbial growth and 72 hours in total for fungal growth.

  • Bacterial/Microbial Growth: After 30 hours compare the color of sample bottle to the color chart.

  • Fungal Growth: After 72 hours compare the color of the test bottle to the color chart.

  • If it is necessary to dilute samples to be tested, for reasons of viscosity, etc., the following procedure is recommended: A sample may be diluted by adding 1 ml to 99 ml of boiled, cooled tap water in a clean container. Add 1 ml of the test sample to the diluent, cap the container and shake vigorously. Then proceed with the above directions. Take the dilution factor into account when estimating growth. For example, a 105 count from a 1/100 dilution of fluid would indicate 107 organisms per ml in the original sample.

  • STORAGE: Liqui-CultTM should be stored at room temperature (68-75 deg. F). Protect from light. Need not be refrigerated.

  • DISPOSAL OF USED MATERIALS: Under a fume hood fill the bottle with bleach. Allow to stand for 24 hours. Then discard in an appropriate manner. Needles and syringes should be disposed of within local, state and federal guidelines.

Note that in performing testing where a biocide or other similar product has been used in fuel, the test will likely result in a false negative reading for microbial and fungal contamination, but fuel degradation and contamination from other sources is common and the test will not disclose other problems.

Dowload Literature

  Instructions and Comparative Chart


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